How to dissolve lyophilized silk fibroin?

Dear members of the community, I am trying to concentrate silk fibroin to 25 % (w/v). Nevertheless, i am struggling with the concentration step. I successfully achieve concentrations of 5-6 % of...

16 February 2021 1,808 1 View

Does someone have accomplished electrospinning with 15 % PCL using chloroform or dicloromehtane as a solvent?

Dear community, I am trying to conduct electrospinning with 15% of PCL and I would like to do it with chloroform or dicloromethene. When I try to do it I do not manage to form a Taylor Cone that...

06 August 2020 3,823 6 View

Is it dangerous to inhale Lithium bromide?

Hello all, I am diluting LiBr with water and I wonder if I should do it under the fume hood or if it is okay to do it anywhere in the laboratory. Then, if I might do the reaction under the fume...

21 July 2020 7,892 3 View

Monthly to daily time series, R or MATLAB code?

Hi, I have a recent 30-year monthly rainfall time series, and a recent 5-year daily rainfall time series. I am looking for disaggregating monthly to daily time series using statistical...

08 July 2018 3,984 2 View

What Spike Sorting algorithm/platform should I try next (Kilosort vs others)?

Dear all, Is there a Spike Sorting revolution taking place? There is always progress and new ideas on how to sort electrophysiological spikes in order to study single unit activity. I have the...

05 December 2016 2,120 2 View

Does anyone have a good protocol/kit for measuring ROS levels in culture media?

26 April 2016 9,223 7 View

About the HSCL-20 (in Spanish).

I look forward to using the HSCL-20 questionnaire to monitor the evolution of depressive symptoms in a research project. This scale is widely used in US clinical trials on disease management...

13 March 2013 1,705 1 View

Protein adsorption, large variation

Dear members of the community, I am building a 15 % PCL scaffolds for cell culture dissolving the PCL with different solvents. I would like to know which one adsorbs more protein. That is why, I...

01 January 1970 9,338 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View