Which surface markers do you use to assess differentiation by PMAin K562?

More Enric Redondo Monte's questions See All

Does anybody know an online server to build phylogeny with more than 50 isolates?

The CSI phylogeny 1.4 no longer allows to build a phylogeny with more than 50 isolates.

28 July 2023 7,615 5 View

¿Conoces el Inventario de Edificios de Interés Arquitectónico de Propiedad Pública de Carácter Local en Andalucía 2021-2022 (IEPIA)?

Ya puedes consultar la publicación del Inventario de Edificios de Interés Arquitectónico de Propiedad Pública de Carácter Local en Andalucía 2021-2022...

20 July 2023 7,518 0 View

How to analyze multiple choice data: resampling (behaviour ecology)?

Hi everyone! I am investigating if different variables are associated with the choice of a nest box in a wild bird population. Briefly, I am comparing the characteristics of multiple nest-boxes...

09 October 2022 5,387 3 View

Looking for a spreadsheet based water balance model for lake operation?

Hi, I am looking for a spreadsheet-based water balance model for lake operation. Please don't refer me to papers. I am looking for an actual spreadsheet. Thanks

31 January 2022 2,965 11 View

Does anyone know how to check if a given coordinate is within a polygon?

Hi! I am currently analyzing prospecting data from a bird population to explore which nest boxes are more prospected and which variables may be influencing this. I have created MCP (minimum...

17 October 2021 6,237 0 View

How to compute Moran's I for multiple years?

Hi, I am checking for spatial autocorrelation in my dataset. It comprises the ID of the nests, the longitude and latitude for each of the nest boxes and the number of fledged chicks for each...

15 June 2021 861 5 View

Can someone help me with problems with Amyloid beta 42 and HFIP?

Hi there, I have purchased Abeta42 from Bachem (1 mg). Usually, I use HFIP (hexafluoroisopropanol) to monomerize the protein, and after this, I always check if it is aggregating over time...

17 April 2021 1,341 0 View

How to dissolve lyophilized silk fibroin?

Dear members of the community, I am trying to concentrate silk fibroin to 25 % (w/v). Nevertheless, i am struggling with the concentration step. I successfully achieve concentrations of 5-6 % of...

16 February 2021 2,153 1 View

Does someone have accomplished electrospinning with 15 % PCL using chloroform or dicloromehtane as a solvent?

Dear community, I am trying to conduct electrospinning with 15% of PCL and I would like to do it with chloroform or dicloromethene. When I try to do it I do not manage to form a Taylor Cone that...

06 August 2020 4,016 6 View

Silk fibroin gels during dialysis. How can I fix it ?

Dear members of the community, I am trying to elaborate silk fibroin. I boiled the silk for 30 min. Then, when it is dry, I cut it in small pieces and dissolved it with 9.3 M Libr solution (I...

05 August 2020 3,195 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Problem with Two-phase simulation?

Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...

25 July 2024 2,309 5 View

Flow through curved domains?

Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...

25 July 2024 9,058 4 View

BD Cell Viability Kit - TO evaporation?

Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...

22 July 2024 2,257 0 View

Can I use CCCP instead of H₂O₂ as a positive control for ROS measurement through flow cytometry?

I am giving starvation stress to the soil amoeba (Dictyostelium discoideum) cells in my study groups and using NAC as a negative control. I am using DHE for ROS measurement.

20 July 2024 6,354 0 View

I came across oscillations in a pressure profile for a pipeline flow along the axis of a cylinder, how do I prove that these are not numerical err?

In terms of CFD, we often analyze the stability of the error using Von-Neumann analysis, especially for FDM based problems. Should we follow the same approach for a compressible fluid flow using FVM ?

13 July 2024 6,296 5 View