Tate MacDonald

3 Questions 1 Answers 0 Followers

Questions related from Tate MacDonald

Proper storage of a western blot membrane following visualization with HRP substrate?

I've heard that storage in TBST is acceptable. What specific temperature should I store at? How long is the membrane viable for?

03 June 2021 5,258 1 View

Troubleshooting in Probing for Myc Tag in HEK Cells?

I've been having issues visualizing blots when probing for the Myc tag in HEK cells. I'll list my basic step outline that I follow, maybe that will be informative in picking out something I'm...

03 June 2021 9,260 3 View

How much protein to load when probing for Myc tag?

I'm probing for a Myc tag expressed in HEK cells. Generally, how much protein should I load? I tried 25 ug last time to no avail (absolutely no signal) so I tried doubling to 50. Should I go even...

01 June 2021 928 2 View