Dear scholars:
I found the proliferation of cancer cells was inhibited by our drug.
This drug did not induce cell death.
We used PI staining to investigate cell cycle distribution.
However, no obvious phase arrest (
Dear I-Lun Hsin,
Could it be that cells are still deviding, but much slower? Your drug could have an effect on cellular metabolism, providing less energy to the cells?
Hello I-Lun Hsin, If you can not see any change in their relative cell cycle distribution - does that include hyperdiploid cells(https://www.nature.com/articles/nprot.2006.238.pdf)? If no evidence of increased apoptosis(hyperdiploid peak and/or annexinV expression) - you could run parallel cultures with/without drug and do comparative daily counts and viability assessment - PI or trypan blue by microscopy, to determine if the cell cycle had been inhibited. Further, you could label cells with a cell tracer eg CFSE, ef450 and track division times and population dynamics. Best wishes
martin
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