We used the dual culture to detect the antagonism of trichoderma , the methods are as follows :
Trichoderma and S. sclerotiorum were seeded in a same Petri dish containing PDA at opposite sides. Controls were performed using S. sclerotiorum against itself. Fungi were grown up to 7 days at 28 C.
The inhibition efficiency was calculated using the following formula:
inhibition efficiency (%) = (C-T)/C*100
( C=growth radius of pathogen in control plates，T=growth radius of pathogen in dual culture plates).
My question is: if the Controls were performed using S. sclerotiorum against itself, how to measure the growth radius of pathogen in control plates if the
S. sclerotiorum hyphae overgrow/pass across each other ?
Hi Wang: As i Know and work with Trichoderma antagonism, I prefer to use scale 1-5, Plese find this paper in the link below:
https://www.apsnet.org/publications/phytopathology/backissues/Documents/1982Articles/Phyto72n04_379.pdf
Hi Wang
I agree with Dr.Oadi
It doesn't prefer to use this quotation because the interaction not describes very clear
94
About the S. sclerotiorum against itself
You use same of Trichoderma - scale 1-5
You can consider this method:
http://www.sciencedirect.com/science/article/pii/S0167701205002903
I see no reason why we should test the growth of Sclerotinia sclerotiorum against itself.
Regards, Franci
137
You are welcome,
See this method "http://www.tandfonline.com/doi/abs/10.1080/03235400903266438?journalCode=gapp20"
Dear Wang,
I do not understand the purpose of the control you were performed i.e. dual culture Sclerotinia against Sclerotinia. Please, if you can explain.
Regards, Franci
Hi, dear Franci,
The control in dual culture, Sclerotinia against Sclerotinia, was used to measure the growth radius of pathogen.
This kind of methods were widely used in dual culture analysis to detect the antagonism of trichoderma. However, I think this method might have some problems as described above. AS Dr. Laith and Oadi put it, scale 1-5 migth better.
Hi Wang,
As I know, in control plate must be only one pathogen inoculum. You can see this in the attached photo, which shows the antagonism between the S. sclerotiorum and the various Trichoderma species (these tests, I worked 25 years ago). Control is in the center.
Regards, Franci
I agree with Franci Aco Celar. It's classical method where in control plate must be only one pathogen inoculum. In addition for Trichoderma strains with active growth is necessary to use the method of "deferred antagonism". In our studies, 7 days was too much. Trichoderma covers the surface of petri dish for 3-4 days.
Dimitry was right. Seven days is too much if you are using Petri dishes with a diameter of 90 mm. Of course, the time also depends on the species of Trichoderma and isolate.
Hello ,
For the control , you just have to put S.sclerotiorum alone and the test will over when the petry dish is full
Best regards
I agree with Dmitry. Though Franci told growth time depends on the species of Trichoderma but yet its enough 4-5 days at 25-30 degree Celsius
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