5 ml of PBS was put into Brdu vial containing 250 mg was dissolved in PBS and set to dissolve first at RT then 37 degrees C, then 50 degrees but after several days of repeating dissolving at 50 degrees, minute quantity remains undissolved. Now I am also worried about the integrity of soln. I will use it to check lymphocyte proliferation after stimulation with antigens
I have used BrdU from a BD kit, it is supplied at 10mg/ml (32.5mM) diluted in DPBS. This stock solution is stored at -80oC. Perhaps your BrdU (50mg/ml) is insoluble because it is too concentrated? I would try re-constituting at 10mg/ml instead of 50mg/ml if possible. If concentration is not the problem you could try re-suspending in DPBS (without calcium chloride) rather than PBS it might help.
I would not be confident of the integrity of your BrdU solution after reheating to 50 degrees several times.
Try adding a few microlitres (or drops) of tissue culture grade DMSO to your 250 mg BrdU powder, just enough to dissolve all of the BrdU. Gradually add the 5 ml of PBS to the BrdU DMSO. You shouldn’t see any un-dissolved BrdU.
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