Hallo everybody,

I was extracting RNA (with TRIzol protocol) from bees in RNAlater. At the precipitation step we found that the aqueous phase was not mixing with the isopropanol - as if they were "oil and water". Looking through the internet I found that if this problem occurs- "simply add a mixture of 50% water, 50% isopropanol until the solution becomes clear and the two phases mix. The amount of water/isopropanol required will depend on how much RNAlater was carried over; if the sample was mostly RNAlater, as much as an equal volume may be needed.".

Doing so we end up adding more then two times volume but the two phases did not mix...

Did something like this happend to anybody? does anybody have a solution to this type of problem?

Would love to hear your thoughts,

Tal.