Where can I get "whole organism" mRNA expression levels (of many organisms)?

Hey, I need to get the mRNA expression profile of multiple organisms, and I do not need it to be specific for a certain tissue or status, but rather the average values per organism. For example,...

09 December 2020 3,620 3 View

Best way transfecting two sgRNA's on two different CRISPR/CAS9 plasmids?

I'm trying to knock out a gene in mESCs. I have my guides already cloned each into a CRISPR/CAS9 plasmid also carrying GFP. My guides didn't work after transfecting them individually, so I wish to...

03 November 2020 6,468 3 View

Which sensory-motor learning task and problem solving task are well-researched and easy to administer (do not require special & expensive equipment)?

We are planning a study in which we will explore the effects on the brain of a sensory-motor intervention which simulates the process of developmental sensory-motor learning. We plan to explore...

27 December 2018 6,935 1 View

Which resources could help residents develop a successful grassroots participatory process ?

In my article "When knowledge is power: grassroots participatory initiative as a process of resource development" I claim that residents are initiating informal grassroots participatory...

25 December 2017 1,657 12 View

Is slashing and mulching of Asparagus leaves at the end of the growing season more efficient than allowing nutrient translocation to take place?

In Australia, commercial asparagus farms cut back vegetative growth and mulch it into the soil at the end of the growing season in order to return the nutrients in these plant parts to the soil....

19 November 2017 988 3 View

Can any one give me a protocol for beta gal ONPG assay for AML12 transfected cells?

I have a protocol which we used a few years ago with 3T3 cells. With the AML12 cells I don't get any color. Any suggestions? Thank you.

30 October 2017 1,749 1 View

How to merge files and define new values on SPSS?

Well, I'm facing some troubles with SPSS. I'm trying to merge 2 files and I will try to explain the problem with a simple example: Let's say I have names of patients in the "master" file, while on...

14 September 2017 5,619 3 View

Which statistical model(s) is this paper using?

Differential tolerance to metals among populations of the introduced bryozoan Bugula neritina (Piola and Johnston 2006) I have been asked to review this paper and comment on the statistical models...

16 August 2017 9,131 3 View

Problem mixing the aqueous phase with isopropanol - RNAlater samples

Hallo everybody, I was extracting RNA (with TRIzol protocol) from bees in RNAlater. At the precipitation step we found that the aqueous phase was not mixing with the isopropanol - as if they were...

21 June 2017 6,379 1 View

Besides T7 RNA Polymerase, is there any other protein that binds the T7 promoter?

I know that T7 RNA polymerase does not require transcription factors , but is there any other protein besides the polymerase who can bind to the same promoter?

10 January 2017 8,049 2 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

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25 February 2021 5,713 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How to search for crystal structure for material with atomic position labelled?

I am using the crystallographic open database and materials project to search for cif file but the exact structure is not mentioned or how can I deduce that the given structure and atomic position...

23 February 2021 6,723 2 View