11 April 2021 2 4K Report

I am calibrating my Gilson pipette following the manufacture's instructions - the adjustments are done when the pipette is on low volume (i.e for a P1000 pipette I'm doing the adjustment while on 100ul).

After getting to an accurate measure in low volume I'm checking the accuracy at the high volume and it's not accurate at all. If I try to adjust in high volume and then check the accuracy of the low volume its again not accurate.

I have read several formal instructions on how to calibrate a pipette and all of it mention checking high and low volume for accuracy while doing the adjustments on low volume.

Will appreciate any tip or suggestion how to successfully calibrate my pipettes both in high and low volumes...

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