Hello,
I have been trying to validate the presence of serum and plasma exosomes using WB with TSG101 as an exosome marker since a very long time, but with no success. I use the whole cell lysates as positive control and the serum and plasma exosomes at different concentrations. I am able to observe a lot of IGg heavy and light chain bands because of which I am not able to figure out if my samples are actually showing the expected molecular weight or are just migrating with the heavy chain bands. How do I get rid of these non-specific bands? Any suggestions and inputs would be very helpful.
Thanks,