Primer designing for wheat

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How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

How to model rupture in geogrid in abaqus?

Hi researchers I wanna model tensile test on geogrid in abaqus, so I added displacement to one side of geogrid and the other side of geogrid is constrained completely, everything seems normal but...

19 February 2021 2,195 2 View

Any methods to train a neural network with random (stochastic) and non-measurable variable?

Dear scholars, I was wondering if there are any scientific methods to train a neural network or ANFIS when one of the input variables is stochastic (random) and more importantly non-measurable?...

17 February 2021 1,909 3 View

How to generate MATLAB code from ANFIS model?

Dear scholars, I was wondering how can I generate MATLAB code of an ANFIS (FIS) model? I need to integrate my ANFIS (FIS)/Fuzzy logic system into another MATLAB code to render optimizations. Do...

04 February 2021 6,098 4 View

In the tensile test for a nanocomposite sample, if the sample width decreases (thickness is constant), what does the stress-strain curve change?

Given that the stress is equal to the force divided by the area(width*thickness), what change does the reduction in sample width make to the stress-strain curve? Does stress change occur...

09 January 2021 5,124 5 View

How to convert Shamsi dates into Christian dates in SPSS?

Hi I have a database in which there are several dates indexed as strings and in Shamsi format. I need them as dates (not strings) and prefer them to be Christian. I have tried "Date and Time...

14 December 2020 2,798 3 View

Does anyone know a free large dataset from smart factory with data collected from IoT objects that I can use in academic research?

I am working on the integration of the Internet of Things into business processes. To evaluate my method, I need a large dataset with data collected from different kinds of objects. Thank you!

08 December 2020 7,338 4 View

Can anyone help or advise how and by what mechanism graphene oxide-activated persulfate can react with acrylamide and polyacrylamide in water solvent?

What are the products of the reactions mentioned above? And by what mechanism are they produced? Does graphene oxide itself participate in the reaction? Does not sulfate react with graphene...

18 November 2020 6,938 6 View

How to simulate locking range of an injection locked frequency doubler in cadence ?

Hi im trying to simulate an injection-locked frequency doubler (15 to 30 GHz) in cadence, I'm plotting DFT of transient signal to calculate injection locking range or to see injection pulling...

14 November 2020 896 4 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View