Primer designing for wheat

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What do you know about classroom management?

Hello, if possible, please send me new methods of management and communication in the classroom. thank you؟

18 June 2024 4,139 3 View

How do we measure the electrical conductivity of this magnetic nanocomposite?

A magnetic nanocomposite has been synthesized and its electrical conductivity needs to be measured. What analysis and technique can be used to measure the electrical conductivity of this magnetic...

16 June 2024 7,488 3 View

Is it possible to estimate Horizontal in-situ stresses' directions and magnitudes from seismic data by using AI methods?

We know that one of the important members of a 3D Geomechanical Model is in-situ stresses' directions and magnitudes. we can use the seismic data to estimate these parameters in special record...

21 May 2024 1,332 2 View

Hello. why do we measure surface tension?

surface tension

20 May 2024 4,895 1 View

What are the S-scheme and Z-scheme heterojunctions in photocatalytic systems? for the types of heterogeneous photocatalysts, how can you determine?

what are the S-scheme and Z-scheme heterojunctions in photocatalytic systems? and for the types of heterogeneous photocatalysts, how can you determine the type of S-scheme and Z-scheme?

16 May 2024 1,545 1 View

Why is my first article being rejected fast by related journals?

Hello everyone, I hope you are all doing well. I have already checked plagiarism, related articles’ structures, and grammar, and they seem fine, but good impact factor journals that I have written...

15 May 2024 1,007 4 View

Altitude control of a quadrotor ?

hi there i'm working on quadrotor path tracking in matlab's simulink . there is an inner loop which is responsible for tracking the desired euler angles generated by the outer loop . feedback...

05 May 2024 7,705 5 View

How to perform LC-BLYP calculation with GAMESS?

Hello Dears, I am currently working on time-dependent density functional theory (TDDFT) calculations for both singlet and triplet states using the LC-BLYP functional in GAMESS. I believe I have...

01 May 2024 4,614 0 View

Does Baicalein interact with DMEM high glucose or FBS?

greetings, I'm culturing SW480 cells on DMEM high glucose gibco with FBS. When I'm performing MTT Assay, upon treatment of cells with Baicalein, the color of medium with 5% FBS changes to dark...

25 April 2024 7,115 4 View

I'm getting error:( Domain error. To compute complex results from real x, use 'sqrt(complex(x)) ) in simulink?

hi there . i'm currently working on a thesis which involves designing a fast nonsingular terminal sliding-mode controller for attitude control of a quadrotor. the outputs of sliding mode...

20 April 2024 425 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

How to Input and Analyze Results for an Asymmetrical Factorial Design in STATISTICA 13.3?

In STATISTICA 13.3, I used the Experimental Design Builder under the Design of Experiments tab to generate a design matrix for an asymmetrical factorial design (1443) with 12 experiments. However,...

29 July 2024 4,155 2 View

Pragmatic inquiry research design?

Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...

28 July 2024 540 2 View