Primary cell culture

09 September 2012 1 2K Report

Can anybody provide a detailed protocol for bovine smooth muscle cell culture, from tissues? If we brought the tissue/organs from slaughter house is primary culture possible?

Rakesh Roy

Dear Soni

check this article for details....

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT

Volume 14, Number 12 (1978), 966-980, DOI: 10.1007/BF02616210

check this link for general protocol:

http://www.biorad-ads.com/09-0804_educational_tools/09-0248_Bio-Rad_Basic_Cell_Culture.pdf

All d best.

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