Calmodulin assay - FAQS.TIPS

More Soni Shaikh's questions See All

Can speed change your direction?

Light always follows a straight line during its propagation. I think everything, either a particle or wave having equal or higher speed than light must follow a straight line. Is there any...

03 April 2014 540 3 View

Matrix metalloproteases (MMPs) on physical exercise

I am interested in investigating the role of physical exercise on MMPs and it is well-known that after over exercise MMPs (mostly MMP2 and MMP9) expression increases. My interest on the cellular...

10 November 2012 6,035 3 View

Denovo modeling for protein fragment

I submit the peptide fragment of 30 aa (pdb is unavailable) in robetta and ITASSER for structure prediction, I got the data from ITASSER but not from robetta (still in line) from over 30 days. But...

10 November 2012 3,347 15 View

How much GO6976 for PKC alpha inhibition?

GO6976

09 October 2012 8,547 3 View

Primary cell culture

Can anybody provide a detailed protocol for bovine smooth muscle cell culture, from tissues? If we brought the tissue/organs from slaughter house is primary culture possible?

08 September 2012 2,074 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

Soni Shaikh

I am still waiting for your kind reply, please anybody......

Danton H O'Day

Hi Soni, I don't have a specific protocol for what you are aksing but I'm sure you can find one online or in a published articel. You can purchase calmodulin antibodies to detect CaM via western blotting, ELISA and a host of other methods. Dan