Preservations of rabbit corneas?

More Manish Issar's questions See All

How to compare two group with small sample size (n=3)?

I am working on some physiological traits of plant to which its expensive to raise the sample size beyond n=3. so kindly suggest some test which is applicable to test such group(2)

04 February 2021 461 11 View

How or from where can I get energy load profiles for the household, tertiary and industrial sectors specifically for EU27 countries?

Dear Researchers, I am looking for time series or a function to generate yearly, monthly or daily load profiles for the sectors such as household, tertiary and industrial. These load profile must...

11 January 2021 2,307 2 View

Why HIT PV modules have a lower degradation rate compared to multi-Si PV modules under humid environment?

Dear researchers, Greetings! Kindly provide me the explanation that why HIT PV modules have a lower degradation rate compared to multi-Si PV modules under humid environment? I have noticed that...

11 October 2020 6,410 2 View

What is the permissible limit of dye in industrial discharge?

what is the permissible limit of leather industrial dye? I will try to search the paper related to this topic but don't found.

20 September 2020 6,249 3 View

Is it necessary to construct calibration curve of drug in plasma for in-vivo pharmacokinetic study?

Is it necessary to construct the calibration curve of the drug in plasma for in-vivo pharmacokinetic study? Can we use simple calibration curve of drug constructed in solvent not in the plasma...

30 June 2020 6,949 5 View

Can anyone suggest a solidifying agent suitable for human consumption?

To make a food powder into porous solid.

15 June 2020 2,363 2 View

Which solar cell technology is suitable for 400nm-1660nm diffused radiation ?

which solar cell technology is more efficient for 400nm-1600nm diffused solar radiation and why?

27 April 2020 3,969 3 View

Is there any mathematical model to study the electric fringe field for parallel plates having equal and opposite potential?

Some Mathematical expressions will be helpful.

26 April 2020 8,000 2 View

Does anyone know whether NCI-H522 and NCI-H1437 lung cancer cell lines grow as xenograft in nude mice?

Please let me know whether any body in this forum has first-hand experience growing them in nude mice. I was thinking about injecting 5 million cells in 1: 1 ratio of Matrigel and PBS. Let me know.

07 February 2020 4,661 1 View

What width to thickness ratio we need maintain for tensile specimen (Plate type - with dimension lesser than subsize specimen mentioned by ASTM E8 ) ?

I need to calculate tensile properties of aluminium cast alloy (FSW) samples with following dimensions, GL= 10mm....(40mm total length) Width = 3 mm... In order to study the joint properties...

16 December 2019 10,006 1 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

MHC Rabbit Genome Annotation? Domain annotation of each MHC Rabbit Gene?

I want to find a database(s) or a method where I can find and bulk download all the rabbit MHC genes and domain annotation. In humans using Ensembl, all MHC genes have names and domains but in...

28 January 2021 7,722 2 View

Does anyone know a good antibody anti c-Fos working on fish ?

I know this has been asked before, I repropose the question hoping to get an up to date fedeback. I have already tested the E-8 and 6-2H-2F from Santa Cruz with really poor results. Unfortunately...

19 January 2021 6,270 2 View

How to preserve wet fungus spore ?

I need to preserve broth culture of wet fungus spore for my further research. Can any one suggest me any solution.

18 January 2021 4,756 2 View

Does long term preservation (10years) of Collembola in 99% Ethanol affect recovery rate of extraction by flotation?

Hello! I am currently developing an automated counting approach using Fiji to count Collembola from pitfall trapped samples that have been stored in Ethanol (99%) since 2010. I use flotation (tap...

13 January 2021 659 2 View

Can we use two antibodies generated in same species for indirect Sandwich ELISA?

I have generated two antibodies against two peptide fractions of a protein in the rabbit. I want to use these two antibodies (Ab-1 and Ab-2) in indirect sandwich ELISA for the detection of...

11 January 2021 1,754 4 View

Matlab: train random forest with time series? How to transform time series data to a supervised learning dataset?

Hey, I've build random forest using Matlab Machine-Learning Toolbox Function (treeBagger). I've computed several kinematic features like velocity or acceleration as predictors (24 predictors) for...

22 December 2020 2,638 3 View

Why some basidiomycete cultures died quickly when stored at 5C on plate or slopes?

Why some basidiomycete cultures died quickly when stored at 5C on plate or slopes? and is there any method to return them back to life ? specially if the culture produce anamorphic stage ? and...

09 December 2020 4,631 3 View

Sahil Thakur

Old but relevant papers. From what they say you can use K sol or Optisol like human corneas, no need to deswell or use closed state systems.

Jpn J Ophthalmol. 2002 Jul-Aug;46(4):377-83.

Development of novel corneal storage medium: first report. Examinations of rabbit cornea.Tachibana A, Sawa M.

Muneeb Faiq

Hi! Though I cant answer this question well but I am interested to know. I think Sahil has uploaded a good paper. I was wondering if you are planning to preserve rabbit corneas, do you plan to transplant them of use them for any other purpose.

If you want to keep the biochemistry and physiology as viable as possible for, may be, a transplant, then rabbit serum (preferably autologous) with certain antibiotics and added nutrients should work. There are, however, commercial media also available.

Khalid Al-Salhie

Check this link

https://www.ncbi.nlm.nih.gov/m/pubmed/17173742/

Hope you good luck

regards