Hello,
I need to prepare 50mM sodium acetate buffer, pH 5. I figured that I need to prepare 50mM sodium acetate (adding around 820,3 mg to 200ml water) and then add 50mM acetic acid (prepared by adding 1,43ml glacial acetic acid to around 500ml water) to adjust pH to 5
Are these calculations correct?
Make a small 50 - 100 mL glass container of 50% acetic acid by equal volume parts glacial acetic acid and equal parts water
Find a pH meter, calibrate the probe with a pH 7 and pH 4 NIST standard solutions. Dissolve 820.3 milligrams of sodium acetate in about 140 mL of water with a stir bar. Hold the pH meter probe in the solution. Add dropwise 50% acetic acid till pH is 5. If you overshoot, add dropwise sodium hydroxide concentrated stock solution to get the pH up to 5. Then fill with water to final volume 200 mL and adjust the pH with a few drops if it drifts with dilution.
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