Pre-running discontinuous SDS-PAGE gels

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What are the recent research topics in combinatorial design theory related to magic rectangles?

Magic squares/latin squares

01 May 2024 8,098 0 View

About newly developed questionnaire tool ?

Can a newly developed questionnaire tool that is validated and checked for reliability be communicated to an Open Access journal?

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How to add Graphene material in sentaurus TCAD ???

where to get the material properties that need to be inserted in the .par file

08 February 2024 7,961 0 View

How does polarity affect welding? Whast is the arc physics (in detail) behind this phenomenon?

I am getting conflicting information from the web. Some say it is one way for arc welding and another way for tig welding and mig welding. I need some clarity as to how arc physics works. There is...

05 February 2024 3,017 2 View

Is there any method available for saponification of Paprika?

Is there any method available to saponify the coloring pigments in Chilli?

09 November 2023 800 0 View

Is there any recommended voltage/current to run a 18% SDS-PAGE gel?

To briefly explain my experiment, I add a chemical probe to the protein lysate, the probe covalently binds to several proteins in the lysate and further the probe is tagged to a fluorophore by...

12 October 2023 5,176 0 View

A typical silicon based transistor's Base-Emitter threshold is 0.7 Volts. What if we dope p type with silicon and n type with Germanium or vice versa?

Diving into to the basic concept of semiconductors, we know the Base-Emitter voltage for a typical silicon based transistor is 0.7 Volts when both p type and n type are doped with silicon. What...

31 July 2023 3,628 1 View

Is there any research papers available on yoga effecting on incretin hormone levels?

how the yoga increasing incretin hormone levels along with mechanisms?

28 May 2023 3,164 3 View

Is there any research papers available on the effect of yoga on epigenetics?

how yoga is affecting the epigenetics and is there any mechanisms available?

19 May 2023 4,114 2 View

How to do optimization using linear programming in Integrated Farming Systems?

Optimization is a statistical technique and done by using linear programming. it is being used in farming systems.

11 April 2023 6,647 5 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View