Pre-running discontinuous SDS-PAGE gels

More Karthik Shanbhag's questions See All

What will be the effect on leakage inductance in the planar transformer for the LLC converter design?

I have analyzed on the effect on leakage inductance for the planar transformer, therefore came across the research paper and found the below formula for leakage inductance calculation: Also, i...

25 October 2020 8,871 3 View

Can we convert the graphene oxide films into rGO films without actually removing graphene oxide from the substrate?

What are the possible ways to convert GO to rGO?

19 October 2020 2,875 4 View

Suggest me a best filamentous m13 vector to generate scfv using phage display method?

i went through many literature to find out the exact viral vector but it was generally mentioned m13 vector in the papers not specific about the name, can anyone suggest me which m13 vector can...

18 October 2020 2,644 1 View

How to prepare MoS2 nanoparticles and 2D material using chemical method?

How to prepare MoS2 nanoparticles and 2D material using chemical method? Is MoS2 a transparent material, what about the absorbance of the MoS2?

12 October 2020 1,874 3 View

How to rank the random effect coefficients of an independent variable in presence of cross-level interactions in a Hierarchical Model?

I'm building a model that estimates the effect of a firm level variable (x) on its financial performance (y). It is a cross-classified three-level model with yearly measures of a firm in level-1...

03 October 2020 6,187 2 View

Can we detect neuroprotection, ischemic tolerance, ischemic pre conditioning using fmri ?

I want to know whether there are any non invasive techniques to detect neuroprotection in humans

02 October 2020 5,896 3 View

In substitutional solid solutions, do the substituting atoms form chemical bonds as they replace the atoms? How are they replacing the existing atom?

I do not understand how atoms replacing the base metal in substitutional alloys actually replace the base metal atoms and not form bonds.

20 September 2020 433 3 View

How to acheive collimate light rays using the LED Spot light?

I am using the light source as LED spotlight 50W (MFG part no: MASTER LED ExpertColor LED MR16 ExpertColor 7.2-50W 927 10D ). Hence i am planning for lens mechanism to convert the divergence...

24 July 2020 9,527 2 View

Can anyone please help me out an easy method to determine resistant starch?

Dear Researchers, Greetings! I have seen many methods to find out resistant starch content however, I couldn't find easy to follow and reliable methods to determine resistant starch...

28 August 2019 6,668 4 View

Is there a Solution manual for surface water quality modelling book by chapra?

I am referring the book surface water quality modelling by Chapra. I m looking for answers to the unsolved problems given at the end of each chapter. Is there a Solution manual available for the...

06 August 2019 7,322 1 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View