How can I prepare a potassium phosphate buffer 50mM at pH 6 using potassium dihydrogen phosphate and disodium hydrogen phosphate?
Handerson Hasselbach equation:
pKa of phosphoric acid for pH 6 is 7.21
pH = pKa + log ([A-]/[HA])
Now, for A- you put Na2HPO4 concentration, and for HA you put KH2PO4 concentration:
6 = 7.21 + log(A/HA)
log(A/HA)=-1.2
A/HA = 0.063
then, HA+A=0.05 also A/HA=0.063
HA=0.047M
A=0.003M
in one liter of solution.
for molecular weights: KH2PO4==136; Na2HPO4= 23*2+96=142Thus, 0.047*136=6.392g of KH2PO4 and 0.003*142= 0.426g of Na2HPO4 should be added.
Thank you very much Dr. Hatem. That was very helpful.
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