Hello everyone. I would like to coat coverslips/plates with poly-D-lysine. But there are so much different protocols about incubation times. Do you know standard protocol?
Currently I am working on my master thesis. I have a moderated-mediation model, so used process macro model 7. However, when I did a check with Two-way Anova to check if the significance from...
28 November 2023 8,609 7 View
Dear researchers, Could we use immunohistochemistry method to somehow specifically stain the pathologic change of mitochondria on NGF-PC12 or DRG cells? Is there any specific markers for...
28 January 2023 3,101 2 View
Hi, In order to measure the LDH level in the experimental groups in Pc12 cells, I tried the lactate dehydrogenase activity assay kit (MAK066-sigma). I used one on the brand's page for the...
20 November 2021 10,019 3 View
Hi, I applied on PC12 cells 100 ng/ml NGF and incubated for 72 hours to differentiation. After 72 h, approximately most cells differentiate and then I applied an anticancer drug but all of the...
13 September 2021 4,176 6 View
There are various environments where sulfate-reducing bacteria exist. In what kind of natural environments can we find them?
26 November 2020 9,071 8 View
I have conducted an experimental design on exploring the effectiveness of mindfulness skills on students' psychological wellbeing. I aim to test if there is a significant change on students'...
26 September 2020 8,369 3 View
I have converted iPSC to cardiomyocyte and I would like to make functional assays to cardiomyocyte so I need to passage them. If I used accutase for this passage, does it cause any damage to cells...
02 October 2019 7,169 6 View
I am trying to understand the interaction between a protein and material mixture and I expect to see electrostatic interactions. Thanks.
03 June 2019 5,038 1 View
I assessed psychometric features of a construct and after exploratory factor analysis, more than half of the items were excluded. How the construct/ content validity was influenced? Any ideas or...
01 January 1970 913 5 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View