I need to extract different protein fractions in P. biglobosa, I request reliable optimized protocol that allows for efficient albumins, globulins and particularly glutelins fractions in the protein sample.
Crushing your sample with liquid nitrogen gives good recovery of proteins
depending on your sample size you can use a beadbeater as well, like a fastprep
it is an academic writing
21 June 2024 8,384 0 View
Extraction and Characterization of Allanblackia floribunda seed oil for production of Biodiesel answer this question.
31 May 2024 2,153 4 View
Good day, I am Ayo, from Nigeria, a Post Graduate Student in University of Ibadan, Nigeria. I am presently working on my M.Sc. Thesis, (optimal placement and sizing of PV-DG and BESS in a radial...
13 November 2023 7,020 2 View
I am trying to detect the expression of BDNF in hippocampal tissues with western blot via the chemiluminescence method; however, I noticed that only pro-BDNF bands were present at approximately 37...
24 October 2023 7,336 0 View
I am working with DF-19 iPSC, I noticed that my cells attach just fine on vitronectin-coated plate on the first day. However, a day after, I noticed that my cells undergo massive death. I have...
04 June 2023 3,046 7 View
We aim to express the green fluorescent protein (GFP) in cultured neural stem cells for calcium imaging studies. Which of the transfection methods (electroporation or lipofectamine) is highly...
12 May 2023 1,020 0 View
I am entirely new to cell culture studies and I need clarification on determination of seeding number after cell count with Neubauer hemocytometer. Say I have a cell density of about 4x10^6 and I...
08 March 2023 1,343 2 View
in resource limited settings, the may be need to carry out platelet function test without the use of equipment. which is the best technique and is the result comparable to standard platelet...
09 April 2022 7,270 3 View
I conducted interview as part of a survey with the view to reporting my findings through qualitative analysis. However, part of the interview questions are demographic (age, sex, education,...
27 October 2021 746 4 View
What is the best approach to induction of anemia in experimental rats?
15 September 2021 4,154 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am new to Micromechanics and having similar problem with understanding the implementation of the formula's. I would appreciate if anyone can guide me on how to go about getting a scalar value...
30 July 2024 969 0 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View