We aim to express the green fluorescent protein (GFP) in cultured neural stem cells for calcium imaging studies. Which of the transfection methods (electroporation or lipofectamine) is highly efficient and will also maintain cell viability?
Extraction and Characterization of Allanblackia floribunda seed oil for production of Biodiesel answer this question.
31 May 2024 2,153 4 View
Good day, I am Ayo, from Nigeria, a Post Graduate Student in University of Ibadan, Nigeria. I am presently working on my M.Sc. Thesis, (optimal placement and sizing of PV-DG and BESS in a radial...
13 November 2023 7,020 2 View
I am trying to detect the expression of BDNF in hippocampal tissues with western blot via the chemiluminescence method; however, I noticed that only pro-BDNF bands were present at approximately 37...
24 October 2023 7,336 0 View
I am working with DF-19 iPSC, I noticed that my cells attach just fine on vitronectin-coated plate on the first day. However, a day after, I noticed that my cells undergo massive death. I have...
04 June 2023 3,046 7 View
I am entirely new to cell culture studies and I need clarification on determination of seeding number after cell count with Neubauer hemocytometer. Say I have a cell density of about 4x10^6 and I...
08 March 2023 1,343 2 View
in resource limited settings, the may be need to carry out platelet function test without the use of equipment. which is the best technique and is the result comparable to standard platelet...
09 April 2022 7,270 3 View
I conducted interview as part of a survey with the view to reporting my findings through qualitative analysis. However, part of the interview questions are demographic (age, sex, education,...
27 October 2021 746 4 View
What is the best approach to induction of anemia in experimental rats?
15 September 2021 4,154 4 View
I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...
19 February 2021 3,155 2 View
I need a complete &chem options that will enable me simulate SOA over west Africa. I have already chosen the dynamic, physics and other options. I shall be grateful for any help.
30 August 2020 8,084 2 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds
04 August 2024 3,019 3 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...
01 August 2024 9,227 1 View