We aim to express the green fluorescent protein (GFP) in cultured neural stem cells for calcium imaging studies. Which of the transfection methods (electroporation or lipofectamine) is highly efficient and will also maintain cell viability?
I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...
19 February 2021 2,482 2 View
Using STATA , I am having issues detecting what the R square and Adjusted R square from a generalized least square for which standard error estimates are robust to disturbances of...
12 February 2018 8,688 2 View
Vector + Insert = almost 12kb Vector:Insert ratio used; 1:2, 1:3, 1:4 Controls: Vector + Ligase only, Uncut Vector w/o ligase Ligation: O/N at 16 C Transformation: About 2ul into DH5alpha...
01 June 2017 893 3 View
I need someone to help me with the steps I will follow to simulate red blood cell in a unit cell of Deterministic lateral displacement device through comsol multiphysics.
27 April 2015 6,733 6 View
I would like to set up a taqman multiplex qPCR assay. Instead of using different dyes in my probes, I think a quencher can be used for 2 reporter dyes, e.g. FAM -TAMRA and JOE-TAMRA.
31 March 2015 960 3 View
Hello there. I am getting significant activity from my enzyme extract in recent times. But whenever I try to run a 12 % SDS-PAGE for the extract I end up with absolutely no bands. I tried to run...
01 January 1970 4,798 3 View
What are the best markers for evaluating the immunological effects of massive allogenic blood transfusion?
01 January 1970 5,856 3 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
I looking for ferro calcium production process
03 March 2021 1,755 4 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.
02 March 2021 7,735 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...
01 March 2021 6,200 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View