How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

How to define least effective concentration (LEC) and maximum safe concentration (MSC) of drugs?

In drug delivery, for efficacy, the drug concentration needs to be within LEC and MSC. How can we define that concentration?

25 November 2020 6,478 3 View

Perception on business failure prediction models using the annual report?

27 July 2020 1,141 2 View

Any suggestions for tools to analysis, plotting and tabulating genomic data of bacteria genomes?

Hello, In order to analyse NGS results, I looking for some software or tool to tabulate the entire genome of a bacteria. Thanks in advance

27 July 2020 9,826 5 View

Dissertation Research: Business failure prediction. Could you help me with a short survey please?

I'm conducting a research study titled 'Perception on the business failure prediction using the annual report' to receive the degree of M.Sc. Accounting and Finance by Griffith College in Dublin,...

21 July 2020 8,761 3 View

How much does it cost a Frantz Magnetic separator?

I would like to know the price of a Magnetic sperator to manage the separation of zircons, apatites and heavy minerals. I'm not interested in explanation of other methodologies, i just would like...

09 January 2020 4,590 2 View

Why does a small peptide precipitates in contact with albumin?

06 January 2020 8,673 5 View

Could you help me to find a paper?

16 August 2019 3,725 3 View

What are the reasons for cell viability of more than 100% for DMSO control vehicle in MTT assay?

I performed MTT assay for assessing the cytotoxicity of curcumin (0-50uM) on LPS-stimulated THP-1 macrophages, and used DMSO as control vehicle. While calculating the cell viability% for DMSO, I...

26 June 2018 8,010 6 View

No title

As a control in my MAPK western blots I routinely use the anti-Fus3 antibody from Santa Cruz (SC-6772; YN-19SC), but recently learned that it has been discontinued from the catalogue. The same is...

07 February 2017 2,807 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

The procedure compute?

02 March 2021 5,204 3 View

Does anyone know what a '.' means in SPSS?

Hi everyone! I was wondering if anyone would be so kind as to help me with regards to my data set on SPSS! I was checking for multivariate outliers via the standardised residuals ('Linear...

02 March 2021 7,910 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View