How does the point & click method in SPSS form matching pairs in Propensity score methods?

What method does the SPSS point and click option run for propensity scores? That is does it do matching with replacement or matching without replacement, and does it do greedy or optimal...

17 March 2019 4,975 3 View

Has anyone tried to use Ag as an adhesive layer for thin film deposition?

I currently clean the SiO2 substrate with Piranha solution for 1-2 hours. I've also used Cr and Ti as the adhesive layer and neither have worked. Both samples create thin films that can easily...

02 October 2018 928 3 View

Does anyone know at what concentration DSMZ supply bacterial DNA?

13 June 2018 6,306 2 View

Where has this article been published online? Would you be willing to provide a URL please?

Please provide a link to the online article.

06 June 2018 5,587 2 View

Primary dendritic cell culture?

Hi could anyone point me in the direction of a paper that talks about the length of time that mature (i.e. not BMDC) DCs eg from lymph nodes can be incubated ex vivo before cell death?

12 November 2017 4,691 2 View

Can overlap extension PCR be used to simultaneously insert and remove base pairs?

I want to use overlap extension PCR to insert a domain into my construct. However I have just realised that upstream of the region where I want to insert the domain there are 19 amino acids...

22 May 2017 5,117 4 View

Can anyone advise on now to compare ligand binding sites in NMR and crystal structures?

New to crystallography...I want to compare ligand binding in a crystal structure to one generated using NMR. I have tried the pdbsum and the pisa servers but the hydrogen bonds that are identified...

27 December 2016 1,451 3 View

Does any one have any experience of creating a stable cell line using Calu-3 cells?

I am looking to create a stable cell line using Calu-3 cells. My initial plan is to co-transfect with pBABE-puro and select with puromycin. Does anyone have any similar experience with Calu-3? I...

16 August 2016 3,334 5 View

Do I need to codon optimise my sequence for expression in a mammalian cell line?

My POI is a human protein. However we when we had the DNA sequence synthesized we codon optimised for S.cerevisae (for protein expression). However, I now wish to do some interactome studies in a...

20 July 2016 1,429 3 View

Why would an excess of template DNA cause a smeared band during PCR?

I was undertaking a PCR reaction using a potentially large amount of ss-DNA following a SELEX procedure. I observed a smeared band of DNA. I have read that a large amount of template DNA can cause...

27 April 2016 6,950 4 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

The procedure compute?

02 March 2021 5,204 3 View

Does anyone know what a '.' means in SPSS?

Hi everyone! I was wondering if anyone would be so kind as to help me with regards to my data set on SPSS! I was checking for multivariate outliers via the standardised residuals ('Linear...

02 March 2021 7,910 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View