Lo Tzu Yu

8 Questions 39 Answers 0 Followers

Questions related from Lo Tzu Yu

1Xpbs diluted from 10X pbs stock can alter the ph value?

I am wondering why there are two recipes to prepare 10X pbs, one for adjust ph value to 7.4, and the other one is to let the ph value remain around 6.8 after solving the salts. The latter one...

20 October 2023 2,925 0 View

Seqence remove from LentiCRISPRv2 ?

Hello everyone; I am new to CRISPR. Owning to our strategy to screen positive cells, we yearn to involve two selection markers (GFP and puromycin) in the LentiCRISPRv2 vector. However, we figure...

19 July 2023 8,106 0 View

Basic questions about Chemiluminescent EMSA ?

Hi everyone, I'm new in EMSA. And I found that Thermofisher offers a Chemiluminescent EMSA kit (catalog: 20158；https://www.thermofisher.com/order/catalog/product/20158#/20158). And I have few...

29 September 2021 3,660 2 View

How did the scientists analyze the iron-related genes in hepatocyte?

Are there any papers or conditions that I can fellow to analyze the iron related genes in hepatocyte (HepG2) with iron or iron chelator? thank you

22 February 2018 7,087 3 View

Is it necessary to publish SCI papers for Applying Ph.D. program in the U.S. ?

Hi, I am graduated from the M.S. program at National Taiwan University. I do have some experimental experiences in my M.S. training. I am wondering whether it is necessary to publish SCI papers...

01 January 1970 2,564 3 View

What is the reasons of DNA gel smear?

Hi, I am wondering why DNA smear in this gel. The plasmids extracted from stbl3 are cut with restriction enzymes. Is it possible for overloading, nuclease contamination, or genomic DNA...

01 January 1970 817 3 View

Seqence remove from LentiCRISPRv2

Hello everyone; I am new to CRISPR. Owning to our strategy to screen positive cells, we yearn to involve two selection markers (GFP and puromycin) in the LentiCRISPRv2 vector. However, we figure...

01 January 1970 2,036 6 View

The ph value of 10xpbs can change after 10 fold dilution?

I am wondering why there are two recipes to prepare 10X pbs, one for adjust ph value to 7.4, and the other one is to let the ph value remain around 6.8 after solving the salts. The latter one...

01 January 1970 7,382 3 View