I am determining the alpha amylase inhibition activity of my methanolic extracts by DNSA method. The problem is, my sample absorbance is higher than the negative control which made the final results in a negative value.
As one of the troubleshooting method, I added my sample and DNS reagent to see if there's a possibility that my sample has reducing sugars and after heating it for 5 mins at 100 degrees, the solution developed color which in terms mean that my samples extracts do have reducing sugars which interfere with my sample absorbance for the alpha amylase inhibition.
Can someone tell me if its the right thing to subtract the absorbance (Sample+DNS) from the final absorbance (Sample+Enzyme+Substrate+DNS) to get a corrected absorbance?
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