Nupur Shah

2 Questions 2 Answers 0 Followers

Questions related from Nupur Shah

Please Share Protocol for transformation ~9KB CRISPR Plasmid Using dh5alpha cells?

I have been preparing competent dh5alpha cells in the lab with good competency not excellent. however, have not been able to transform my CRISPR plasmid yet. I am following all the desired steps...

17 July 2023 1,915 3 View

Can anybody give me exact protocol for siRNA transfection using RNAiMAX ? what should be the ratio between siRNA oligos and RNAiMAX ?

I am doing siRNA transfection using RNAiMAX . i am doing it in 10cm plates. i am not sure about the ratio of RNAiMAX and siRNA to be added per plate. The concentration of siRNA is 10nmole (...

24 July 2015 2,615 6 View