Please, I want to know what is happening to my agarose.

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Does it have any effect on biological specimen?

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I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

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Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

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Daniel L Moran

As pH drops, the gel matrix weakens. If possible raise the pH.

Laurence Stuart Dawkins-Hall

Agreed

its too acidic: Agarose is meant to be used with 1 x Tris borate EDTA (TBE) or Tris Acetate EDTA buffer (TAE) which have a higher pH

Intan Rosalina Suhito

Use TAE 1X which as i know the pH of it should be higher than 6.3, then mix with agarose and melt it to make homogene mixture.