Please, I want to know what is happening to my agarose.

More Ubong Akpan's questions See All

Please I need someone to help me. I want to prepare M/15 or 67mM phosphate buffer

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Please what could be wrong if using the same DNA to amplify gives different results.

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Why are my bands weird in this Agarose gel?

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Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

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Laurence Stuart Dawkins-Hall

Agreed

its too acidic: Agarose is meant to be used with 1 x Tris borate EDTA (TBE) or Tris Acetate EDTA buffer (TAE) which have a higher pH

Intan Rosalina Suhito

Use TAE 1X which as i know the pH of it should be higher than 6.3, then mix with agarose and melt it to make homogene mixture.