Please, I want to know what is happening to my agarose.

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Why are my bands weird in this Agarose gel?

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Daniel L Moran

As pH drops, the gel matrix weakens. If possible raise the pH.

Laurence Stuart Dawkins-Hall

Agreed

its too acidic: Agarose is meant to be used with 1 x Tris borate EDTA (TBE) or Tris Acetate EDTA buffer (TAE) which have a higher pH

Intan Rosalina Suhito

Use TAE 1X which as i know the pH of it should be higher than 6.3, then mix with agarose and melt it to make homogene mixture.