It won't express, unfortunately. Bacterial promoters are very different from eukaryotic ones. What promoter you'd use is pretty case dependent so I think you'd need to give us more info on what you're trying to do with it, broadly.

(In case this is useful info in the future: bacterial transcriptional termination and initiation of translation work differently too. It's also very rare for bacterial mRNA to have introns.)

I am sorry to introduce some mess but Kozac sequence is an eukaryotic feature, that is related with translation. In fact, Kozac sequence is part of the ribosome-binding site in eukaryotic cells. In bacteria promoters have usually the so called TATA box, which acts as anchor for the sigma factor subunit of RNA polymerase, and consequently helps to start transcription.

Best

For expressing guide RNA in bacteria you can use T7 promoter or any other promoters that are easily found in bacteria cloning vectors.

Best of luck

Amy Johnson

I would introduce more noise... I disagree of your opinion that Shine-Dalgarno sequence is a Kozac sequence for bacteria, rather the oposite... :)