If someone can help use for understand what are we doing wrong.
Acibind PAI-1 Kit Results, we found that although the Controls and Calibrators performed well according to specifications, several samples showed results of 0. These are not the expected results, as we would have many patients with disabilities which don’t agree with the bibliography - PAI-1 deficiency is very rare. In addition some of them have thrombotic history - more in favor of increased PAI-1 values.
The processed samples were related not only to individuals with thrombotic tendency but also to individuals with hemorrhagic tendency, in the former levels may be increased while in the latter it is important to detect a deficiency of this fibrinolysis inhibitor (PAI-1
Hi Rogerio Barreira , I'm not familiar with that kit. Could you post the spec sheet?
An alternative could be this kit.
https://mol-innov.com/products/active-human-pai-1-functional-assay-elisa-kit/
Hi dear Steingrimur Stefansson
Thank you for your answer, I'm sending in attach the spec sheet.
We don't know why we have such results?
Hi Rogerio Barreira . PAI-1 in plasma samples goes latent with time and will not bind active tPA/uPA.
I suggest you measure total PAI-1 antigen in the plasma samples as a confirmation that you have PAI-1 in your samples, but it has gone latent.