Hello,
what THP-1 differential markers do you use in the Western-blot method? I am looking for proteins that are characteristic of M0, M1 and M2. Do you have something proven and reliable? Greetings
Pupil Core for simultanious recording of a child and a mum and is willing to share experiences?
07 July 2024 9,066 0 View
I was recently performing a great deal of qPCRs on adipose tissue derived RNA. I had experimental samples from sick, and control samples from healthy individuals. I used the same chemistry for all...
04 June 2024 6,924 0 View
For several years now, the procedure of granting a vote of confidence to the municipal executive by the legislative has been in force in Poland. It takes place annually and is not related to the...
16 February 2024 4,769 2 View
I completed the experiment for one of our colaborators (I measured inhibition of an enzyme by 3 compounds). Unfortunatelly compounds were not active in the range of dilutions that I had used (up...
06 February 2024 3,542 2 View
Climate change is one of the most serious challenges humanity has ever faced. To address the problem UN Framework Climate Change Convention was established Climate change education has been a...
07 September 2023 2,621 2 View
I have the lite version of ZEN ZEISS 3.7 software and I can't use the "Image Analysis" option because it is only available in the blue version. Is there another way to count the number of...
26 July 2023 7,426 1 View
Dear Colleagues, which software is in your experience most suitable for analysing semi-structured interviews? Can you give examples of articles where such data were analysed and results...
14 December 2022 9,735 4 View
Hello RG, I have results from Sanger sequencing for validation of multiple variants I have found in my patients cohort, can you please instruct me if there is some sort of open source program...
21 August 2022 1,286 0 View
Dear fellow Researchers, I have a question - can anybody explain, show literature sources or in any other way guide our team in the complex topic of mechanisms of uptake of organic contaminants...
08 August 2022 4,974 2 View
Has anyone worked with the ReNcell VM Human Neural Progenitor Cell LIne line? I am looking for any protocols/guidelines/tips to help me with my experiments. I am particularly interested in...
23 May 2022 9,582 0 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
15 July 2024 2,055 3 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...
14 July 2024 9,324 2 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,165 1 View
Hello! I am planning a western blot experiment, and I am looking for GAPDH antibodies to order. In the past, I have experienced a lot of hit-or-miss when ordering antibodies based on just the...
07 July 2024 1,685 0 View