How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Does anyone use the ISE electrodes (ammonia, nitrate etc.) to characterise soils samples and plants extracts? What are advantages & disadvanteges?

I will have numerous soil samples/rice rhizosphere sample to characterise as part of the rice microbiome study. I was told that there are ISE electrodes that can be used to measure ammonia,...

19 July 2020 2,066 3 View

Which anti: GRP94 antibody do you recommend?

I am working with C.elegans strain which expresses H.s. GRP94 protein. I am looking for proper antibody to detect GRP94. Do you have some recommendation or suggestions?

13 September 2016 7,214 1 View

Do I need phylogenetic correction to my analyses when I have in data base 11 species?

Dear all, I am performing different analyses (ANOVA, ANCOVA, correlations etc.) and I am not sure if I need phylogenetic correction. On my dataset I have 11 closely related species from Carabidae...

22 November 2015 5,406 12 View

I'm looking for cell dye, which changes it's colour when entering the cell. Can anyone help?

18 October 2015 6,368 3 View

Does anyone have experience with polytypes of CdI2 and their structural calculations?

Or maybe know someone who has experience in this field?

08 February 2015 4,806 3 View

How can I analyse fluorescent images of Double/triple positive cells?

Hi I am using a Columbus for fluo images analysis. I need to define double or triple positive cells. My nuclei are stained with Hoestch (all cells), Sytox (dead cells only) and FITCs (apoptotic...

05 January 2015 3,830 4 View

Does anyone know how to soften the eyeball for histology (paraffin technique)?

02 December 2014 7,688 3 View

Can anyone help me to improve my Lipase assay Kwon and Rhee?

Could anyone help me to improve my lipase assay. I need to study the lipase activity produced by Bacillus saliphilus isolated from alkaline waste lime. I try to apply the method by Kwon and Rhee....

16 September 2014 9,717 29 View

Does anyone know about an ELISA kit that would measure small amounts of human urocortin 2 (UCN2) in serum/plasma?

Detection range around 10-20pg/ml. I have used one but it didn't even detect UCN2 added to the wells. Thanks.

20 May 2014 6,892 5 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View