Hello everyone,
I am facing a frustrating issue with the TC28a2 cell line. After treatment with staurosporine at concentrations of 25nM, 50nM, and 100nM, the cells begin detaching during the first media aspiration when I switch to fresh complete media. This problem occurs regardless of the incubation time. Does anyone have any advice on how to aspirate the media without losing cells?
Hello Rogerio,
In addition to staurosporine treated arms (25nM-100nM), do you also have a control arm that has not been treated with staurosporine ?
Is it possible that staurosporine is simply cytotoxic to your TC28a2 cell line and that’s why they are detaching?
staurosporine is commonly used to induce apoptosis in various cell lines. I’ve used staurosporine for that purpose in HCEC-1CT cell line( human colon epithelial cells) and some tumor cell lines( HUH-7 and NG108-15).
After treatment with staurosporine, cells become round and detach. Under microscope, you can tell that membrane integrity is compromised.
I understand that staurosporine is used as a differentiating agent in chondrocytes. However, there are some literature out there stating that staurosporine can also induced apoptosis in chondrocyte monolayer.
I would simply treat your arms as suspension cells after detachment. Collect them, pellet them and check their viability.
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