Is there a way to get rid of TEA in a TLC plate in order to do proper staining?

Dear colleagues: I have been trying to establish a few HP-TLC methods in the lab. In general, for quantification purposes, I prefer CuSO4+acid charring, to primuline staining. Lately I have been...

20 January 2021 9,019 1 View

Heat and plastic in MTBE lipid extraction?

When extracting lipids with the MTBE method, the indicated temperature is RT. In addition, drying the samples in a speedvac is mandatory due to the presence of water and such procedure is long and...

14 October 2020 9,776 2 View

Increasing yield with DNeasy Power Soil Pro kit?

Hello! I'm extracting genomic DNA from Eucalyptus rhizosphere with PowerSoil Pro kit and the highest yield I'm getting is 3,5 ng/uL max. Also, the quality of DNA is around 1,3, wich is not...

30 August 2020 5,888 4 View

How to overlay and align different scale raster?

I need to overlay two rasters and the pixels of they must be in the same position to each other. I need this to perform a ecological niche model using a package in R. For this end all images must...

20 August 2020 9,503 4 View

How to infer Causality in a observational data?

My Machine Learning model show in different ways, high correlation between parents' education and student's outcomes. I 'm trying to identify a causal relationship in a separate model since the...

11 June 2020 4,186 7 View

Protocol: Single-Locus and Multilocus DNA Fingerpriting, Bruford et al. 1992?

Hi everyone! I know this probably isn't the right place for this, but I have exhausted all other options to access this paper. Does anyone has a pdf copy of the chapter "Single-Locus and...

25 March 2020 2,540 5 View

Are there zinc-CYS parameters in CHARMM files? of has anyone generated these parameters?

06 March 2020 5,939 2 View

Can someone arrange me or indicate where I can find an IR solar panel data set?

I am in a master degree research and I'm in need of a data set for image processing of solar panels with defects (mainly hot spots), so I need to know if someone can help me and tell me where I...

06 January 2020 6,758 3 View

Has anyone had problems performing TRPS analysis of samples purified with TBS instead of PBS?

I work with EVs and I've been facing some issues when doing TRPS (Tunable resistive pulse sensing) analysis of samples that were purified with TBS. All TRPS buffers (Izon Science) are prepared...

20 November 2019 401 1 View

Recommendation on IoT devices for security-related MQTT experiments?

Hello, What IoT devices would you recommend for MQTT experiments? Further recommendations on Smart home devices or simulators for security-related MQTT experiments will also be appreciated.

09 April 2019 8,333 2 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

Which index or sites are reliable in determining of plant species?

There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...

26 February 2021 3,404 10 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

How to calculate battery consumption for IoT device?

Hi, everyone I'm working on a privacy-preserving algorithm that will apply to IoT devices. So, I need to measure the power consumption by implementing this algorithm. Any suggestion, advice,...

25 February 2021 8,749 6 View