Hello dear researchers,
Considering some research on plant tissue culture and induction of callus in order to evaluate and compare the production of a secondary metabolite in the callus with that in the plant in the field,
1) How do you determine how long the experiment and the callus sub-culturing should be continued so that you can come to some valuable conclusions?
2) How do you determine in which increments the samples of the callus and the media should be taken for evaluation of the growth of the callus and the amount of metabolite production?
Thanks in advance for your help
1. Normally we do subculture after 30 days. But there are some plants rich in phenolics, they turn brown so you can reduce the frame. But it mainly depends on the initiation of the shoot tips. You should specify the plant name so suggestions can be more specific.
2. Regarding callus I would suggest take 1g and take liquid media 30 ml in a 50 ml conical flask sealed with stopper. Keep a look on contamination and transfer after 15 days by taking inoculum into a fresh media.
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