Hello,
I'm working on plant tissue culture. Sometimes the tissue I cultivate produces what you can see in the photos attached.
I'm wondering if these are also calluses, or they can be organ regeneration?
Thanks in advance for your precious comments
Dear Elaheh Haghighat it seems early to say something about. However, in my experience, this kind of callus isn't organogenic/embryogenic. In any case, you must wait because explants (these seem petioles) are responding to the treament. Good luck!!!!
Dear Elaheh Haghighat, the ends of the explants show loosely arranged cells due to fast cell divisions in response to the PGRs present in the nutrient solution. It will grow further on the same PGR concentrations because it is not organogenic and embryogenic. Get a good amount of calli first and then transfer callus to lower concentrations of PGRs to regenerate shoots or get somatic embryogenesis. All the very best.
Hi. In my opinion, these are callus, mostly non-organogenic, and would most certainly lead to non-friable nature. However, it seems your media is appropriate, try altering the hormonal combination a bit. Although it is too early to tell, these kinds of calluses often do not lead to anything and would die soon if fine-tuning is not done. If your goal is organogenesis it is disappointing, but if your goal is callus formation it seems to be going somewhere.
Hi, it is difficult to predict your results and where you have to go. For plants regenereration through organogenesis or somatic embryogenesis you have to be certain according your objectives of your research. Should be to explain better?
Thank yoiu
It is like a hairy tissue structure, alike an embryogenic one... Let it go, though and see what happens...
more often desiccating long term cultures with explants show such morphogenesis, sometimes as well with auxins (but dessicating, when the agar gets more solid than semi solid and reducing in swell). You may wish to subculture and see as well other hormones. Yes, as someone already pointed out, these kinds of observations dont lead to further tissue regenerations but stay as is.
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