Target protein was extracted from natural.
And I need to purify it using ion-exchange column chromatography.
Could you please tell me the way to confirm the pI of target protein?
Thanks, sincerely.
Abhigyan Sengupta
Hi,
If the protein sequence is known, then that sequence could be used to find the pI. Several such programs are available online. I found this;
http://protcalc.sourceforge.net/
is very useful.
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Dominique Liger
Hi there,
If the sequence is not known yet and if the protein isn't purified then testing different conditions and different IEC matrix will tell you about the range of pI value.
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