I'm trying to isolate phosphopeptides from flash frozen samples. A sample prep protocol uses sodium orthovanadate as phosphatase inhibitor.
Can I use commercially available phosphatase inhibitor cocktail instead? What is the difference between them?
Vehary Sakanyan
Yes, you can! It is better to use a cocktail (Thermo, Roche or Sigma) as it contains a large spectrum of inhibitors to prevent dephosphorylation of peptides by different type of protein phosphatases (> 200). Check the composition of cocktails to be sure that a protein as inhibitor is not included (probably two cocktails from Sigma are better suited to your goal?). Lastly, use TBS instead of PBS to better conserve phosphate groups in peptides.
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