Sulgi Lee

7 Questions 5 Answers 0 Followers

Questions related from Sulgi Lee

Has anyone successfully cultured oral epithelial cells (Buccal cells) from cheekswabs?

I'm a graduate student and I have collected cells from cheekswabs of donors. I have them in bronchial epithelial cell growth media in adherent T25 flasks. The cells look healthy even after three...

10 October 2017 6,178 1 View

Pre-amplification using TaqMan gene expression assay?

Can I pre-amplify a gene of interest using TaqMan gene expression assay for that particular gene from Thermo? If so, do I have to use their TaqMan pre-amp master mix, which is $900 or can I use...

05 May 2017 1,449 2 View

TF overexpression in IHC and western but gene expression data shows otherwise?

I'm studying a transcription factor in our patient tumor tissues. I have done IHC on about 25 patient tumor tissues vs. adjacent normal tissues. IHC and western shows a significantly more...

11 November 2016 7,844 4 View

Difference between NHS-LC and NHS-SS biotin?

I'm trying to biotinylate surface membrane proteins with NHS-LC-Biotin with Strep-magnetic beads. I'm having trouble with identifying membrane protein with MS (very few identified protein and only...

06 June 2016 5,216 5 View

Why do I have to make sure nuclei is intact when isolating plasma membrane protein?

So I'm learning that in order to increase the yield of membrane proteins by biotinylation and strep-magnetic bead pull-down, I must make sure nuclei is intact after lysis, therefore using...

06 June 2016 4,977 2 View

Phosphatase inhibitors or sodium orthovanadate?

I'm trying to isolate phosphopeptides from flash frozen samples. A sample prep protocol uses sodium orthovanadate as phosphatase inhibitor. Can I use commercially available phosphatase inhibitor...

04 April 2016 1,478 3 View

Would 1% DMSO lyse cells?

I dissolved 100mg of biotin in 1000uL of DMSO. I take 10uL of this and biotinylate cells in 1000uL PBS. This means I have 1% DMSO. Would this cause cells to be lysed?

04 April 2016 5,787 1 View