How to calculate the internal pressure of a riser pipe?

I am designing a riser pipe for use in ocean down to depths of 5km. Polymetallic nodules are pumped from the seabed to the surface. For my FEA and CFD analyses I need to input a maximum pressure...

06 February 2021 2,803 3 View

Looking for an Electronics Engineer working in the field of Materials Science?

Hello, I have an assignment in my material science class that requires us to interview an electronics engineer that practices material science at work. I heard manufacturing engineers at...

16 November 2020 9,318 3 View

Magnetite ultrafine powder supplier?

Looking for a supplier that has 25 micron max particle size as an available option. Found one, but they won't even sell us a sample because they don't think it will work for us. Crazy! All I...

24 August 2020 2,752 5 View

What kind of research topic for industrial engineers take at the time of COVID 19 ?

As we know in this pandemic, everything seems quite hard to control. I hope the researches could discuss and share their thoughts for researching at this time. Especially related in Industrial...

27 July 2020 2,360 7 View

GFP antibody for flow cytometry?

Hello, I have been trying to detect the YFP expression in my YFP-tagged mouse model with anti-GFP antibody for flow cytometry. My question is, is this normal to observe a shift in GFP expression,...

23 July 2020 4,300 12 View

Any recommendations of journals to publish bacterial genome announcements?

I am looking for a journal to publish microbiological/bacterial genome announcements for now and in the future.

17 May 2020 3,010 8 View

Is it okay to use latent variables in the SEM model that have different constraints than CFA for individual latent variables?

I am conducting a structural equation model with three latent variables containing three indicators each. The latent variable CFA's are significant with constraints on 2/3 indicators, but the SEM...

28 April 2020 3,275 4 View

Is secondary fixation necessary for processing plant materials for SEM?

I am trying to create a protocol for several fixation methods for Scanning electron microscopy. using FAA, glutaraddehyde and ethanol as fixatives.

11 November 2019 7,215 1 View

Why won't my freshly isolated primary turkey hepatocytes attach?

We use a collagenase perfusion method to isolate hepatocytes from turkeys. We are culturing them in monolayer with the ultimate goal of using them to evaluate drug metabolism. The experiments were...

03 October 2019 5,662 1 View

What are the stylistic conventions for literature reviews in interdisciplinary papers that use grounded theory?

If you are using grounded theory to extract patterns from your data and you find data that correlates with pre existing theories, is it acceptable to add them to your results and section, or...

09 February 2018 9,160 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View