Hello. I am attempting to cyclize an side chain protected 8mer cyclic peptide from head-to-tail (F to dW). I usually do the cyclization in DMF with PyBOP and DIPEA. I did not get any cyclization. I attempted to cyclize using PyOxim and PyAOP, but they were unsuccessful as well. Any suggestions?
Thanks for the question Samantha. Please give more information such as the concentrations. I have done cyclization many times and I recommend following my paper. The link to my paper is below.
"For the synthesis of cyclic peptides, the protected assembled peptides were cleaved from the resin first in the presence of a cleavage cocktail containing DCM/TFE/AcOH while agitating for 3 h. The side-chain-protected linear peptides were cyclized in the presence of DMF, DCM, DIC, and HOAt overnight."
https://pubmed.ncbi.nlm.nih.gov/35406720/
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