How to calculate the internal pressure of a riser pipe?

I am designing a riser pipe for use in ocean down to depths of 5km. Polymetallic nodules are pumped from the seabed to the surface. For my FEA and CFD analyses I need to input a maximum pressure...

06 February 2021 2,803 3 View

Looking for an Electronics Engineer working in the field of Materials Science?

Hello, I have an assignment in my material science class that requires us to interview an electronics engineer that practices material science at work. I heard manufacturing engineers at...

16 November 2020 9,318 3 View

Magnetite ultrafine powder supplier?

Looking for a supplier that has 25 micron max particle size as an available option. Found one, but they won't even sell us a sample because they don't think it will work for us. Crazy! All I...

24 August 2020 2,752 5 View

What kind of research topic for industrial engineers take at the time of COVID 19 ?

As we know in this pandemic, everything seems quite hard to control. I hope the researches could discuss and share their thoughts for researching at this time. Especially related in Industrial...

27 July 2020 2,360 7 View

GFP antibody for flow cytometry?

Hello, I have been trying to detect the YFP expression in my YFP-tagged mouse model with anti-GFP antibody for flow cytometry. My question is, is this normal to observe a shift in GFP expression,...

23 July 2020 4,300 12 View

How can I roughly estimate the current denisty in an FPGA?

Hello, I am currently working on the estimation of the electromigration of an FPGA. We just performed the construction analysis so we know all the values of wire width and height for each metal...

22 June 2020 2,743 3 View

Any recommendations of journals to publish bacterial genome announcements?

I am looking for a journal to publish microbiological/bacterial genome announcements for now and in the future.

17 May 2020 3,010 8 View

Is it okay to use latent variables in the SEM model that have different constraints than CFA for individual latent variables?

I am conducting a structural equation model with three latent variables containing three indicators each. The latent variable CFA's are significant with constraints on 2/3 indicators, but the SEM...

28 April 2020 3,275 4 View

Can I use Arrhenius Relationship to determine Accelerated factor , when test temperature is lower than use temperature?

Hello, I have a question regarding the acceleration factor. We know that the lifetime (TF) of a component can be expressed as: (1) TF=A0exp(Ea/(kT)) where A0 is a constant (component...

01 March 2020 6,635 6 View

Is secondary fixation necessary for processing plant materials for SEM?

I am trying to create a protocol for several fixation methods for Scanning electron microscopy. using FAA, glutaraddehyde and ethanol as fixatives.

11 November 2019 7,215 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View