How to size battery banks?

I am designing a small grid connected solar PV power-plant. As the battery's capacity is specified in Ah and demand in kWh. What is the best way to size the battery bank? I have battery price in...

28 February 2021 1,638 3 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

What is better to buy a real-time pcr machine or an endpoint?

Hello everyone, we are acquiring a new molecular biology laboratory with the fundamental objective of identifying species of microalgae and cyanobacteria. Perhaps you could help us decide if it is...

24 January 2021 8,320 4 View

How to improve the yield for the reaction below?

I am trying to increase the yield for the reaction below. I did the column and I saw that the major side-product is the symmetric dimer formed by 4-bromobenzyl bromide. I imagine that NaH is...

20 January 2021 7,874 4 View

Is it possible to calculate the energy stored (kWh) in a EV battery by knowing the cathode discharge potential and specific capacity properties?

For example, if I have the following data for a LiNi0,8Co0,15Al0,05O2 compound used in the cathode: - Specific capacity = 200 mAh/g - Average voltage = 3,7 V I have no information about the...

16 January 2021 3,038 2 View

PCR used to work, now it doesn't, why?

I recently did a PCR with certain primers, and the reaction went well for two gels, but when I repeated the exact same reaction a third time it suddenly didnt work, then I did it a 4th time and it...

12 December 2020 364 5 View

What are the parameters that plays a role in life-time degradation models of concrete? what are the most important tests to measure these parameters?

I'm working on a research about the prediction life-time models for concrete in harsh environment. I have to answer those two questions.

14 November 2020 2,043 7 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

How do you annotate figures to show significance?

30 January 2021 1,008 3 View

How can you adjust pH of SM buffer?

Good afternoon, please how can you adjust pH of phage SM buffer? I presume that main ingredient that has influence of pH is Tris/HCl buffer, but according to literature its range of pH is from 7...

27 January 2021 5,767 3 View

What is the composition of TM buffer used for concentrating bacteriophage?

Hello everyone, I have to isolate DNA from Brucella phage , but before that, I have to concentrate the phage using PEG 8000. I have got protocol for concentrating the phage using PEG and TM buffer...

24 January 2021 2,767 6 View

How can I transform 50 kb plasmid into ecoli?

I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...

22 January 2021 8,612 3 View

How an unusual visual effect at the Monte Carlo casino can be obtained?

At the Monte Carlo casino, I saw an unusual advertising image. It rotated around a vertical axis and seemed suspended in the air; there was no screen behind it. The image was bright and colorful....

03 January 2021 9,119 5 View

Bacteriophages very effective against infections. Some bacteriophages can be oncolytic and effective against cancers. How B_P recognize cancer cells?

Bacteriophages very effective against infections. Many of them such as anti-Staphylococcus or anti-Streptococcal widely used in practical medicine. Some bacteriophages can be oncolytic and...

03 January 2021 4,769 1 View

Should i add ATG in the beginning of my polypetide sequence when i want to insert it in a phagemid?

Hi I want to make a recombinant m13 phage displaying my peptide as a fusion peptide on its surface pVIII. Should i add ATG at the beginning when i want to insert it in phagmid vector? Previously,...

18 December 2020 9,931 2 View

Does anyone experience E coli lysis while seed culture?

Hi, I am currently working with BL21(DE3)PLySs and PRSET A vector. Out lab frequently transformation and culture E Coli for protein purification. But recently, even though there is colony after...

13 December 2020 9,167 9 View

Again, what are the bands of extracted plasmid?

I used Trans1-T1 phage resistant chemically competent cells to extracted lentiviral gRNA expression vectors. And there were bands near 2000bp and smear on 1% argrose gel. Did the smear come from...

25 November 2020 858 2 View