I do not know here is phage or not. I incubate over night at 30oC. Vibrio parahaemolyticus causing AHPND and spotting phage on agar.
Please give me advices
I see two issues on your plate. First of all you do not have a lawn on the plate so you won't see plaques without a lawn.
Secondly your "phage stocks" are not sterile and still have a lot of cells in them.
agree with Michael J. Benedik . The bacterial lawn is too thin. Also, the phage suspension is contaminated with bacteria or other cells. Filtering through 0.2um filter paper may solve the contamination problem.
Thank you very much.
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