how to calculate percentage of dye degradation?i have done methylene blue dye degradation by zno nanoparticles, absorbance decreasing as the time passes.i have got absorbance at different time , but i dont know how to calculate percentage of dye degradation?plz help for finding percetage of dye degradation and its statistical analysis?
The absorption spectra of dye must be recorded in terms of change in intensity at λmax of dye.Seperation of ZnO nanoparticles is necessary before spectroscopic analysis. The % of dye degradation is calculated using the following equation:
% Degradation= (Ao - At/Ao) X100
Where
Ao is the absorbance at time t =0 min and At is the absorbance after time t min of treatment . Ao and At are recorded at λmax of dye.
The absorption spectra of dye must be recorded in terms of change in intensity at λmax of dye.Seperation of ZnO nanoparticles is necessary before spectroscopic analysis. The % of dye degradation is calculated using the following equation:
% Degradation= (Ao - At/Ao) X100
Where
Ao is the absorbance at time t =0 min and At is the absorbance after time t min of treatment . Ao and At are recorded at λmax of dye.
For detail, you can read the following article about MB degradation.
http://pubs.acs.org/doi/pdf/10.1021/jp910454r
https://www.insikapub.com/Vol-04/No-01/10IJBAS(4)(1).pdf
https://www.hindawi.com/journals/jnm/2016/9541683/
Hi Sreelekha Sajeesh,
To know the percentage of dye degradation; First, have the data of the initial dye concentration before its action, D0 (time=0 hrs). Then, find the dye concentration reduced during its action at a particular time, Dt (say, t= 2hrs).
% of dye degradation = (D0 - Dt / D0) *100
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