Background information:
Between approximately 2005 and 2014, a company called Invitrogen (later Life Technologies, currently ThermoFisher) marketed special peptides for protein kinase assays. Kinase-catalyzed phosphorylation of these substrates is accompanied an increase in fluorescence intensity, because of a special Mg+2-chelating aminoacid ("Sox" or "cSx") built into each particular peptide sequence [1]. Some researchers who used this assay did publish the sequence of the given peptide. See for example Ref. [2] below, where the sequence of a peptide marketed by Invitrogen under the catalog name "Y12" was Ac-Glu-Glu-Glu-Glu-Tyr-Ile(cSx)-Ile-Val-NH2. Invitrogen, under a license from MIT, sold several dozen of these kinase substrates. There are quite a few scattered papers in the biochemical literature, but unfortunately not all of them list the detailed sequence of the peptide substrates.
Now the question:
Is there anyone who would have in their possession some type of a document (maybe an old Invitrogen product manual, technical note, or any other material) that would list the peptide sequence of each particular kinase substrate marketed by Invitrogen under the Omnia® trade mark? If so, could you please either post that document here, or just contact me directly via email? Thanks very much in advance for your time.
REFERENCES
[1] Shults, M. D., and Imperiali, B., "Versatile fluorescence probes of protein kinase activity", J. Am. Chem. Soc. 125 (2003) 14248-14249.
[2] Schwartz, P. A., Kuzmic, P., Solowiej, J., Bergqvist, S., Bolanos, B., Almaden, C., Nagata, A., Ryan, K., Feng, J., Dalvie, D., Kath, J., Xu, M., Wani, R., and Murray, B. W., "Covalent EGFR inhibitor analysis reveals importance of reversible interactions to potency and mechanisms of drug resistance", Proc. Natl. Acad. Sci. U.S.A. 111 (2014) 173–178.
Ac-EAEAIYAAdP(Sox)G-NH2 for Tyrosine kinases
Article Versatile Fluorescence Probes of Protein Kinase Activity
PKC Ac-Sox-Pro-Gly-Ser-Phe-Arg-Arg-Arg-NH2 C53H80N20O13S 26.7a 413.2 (+3) 413.2 Ac-Sox-Pro-Gly-pSer-Phe-Arg-Arg-Arg-NH2 C53H81N20O16PS 25.7a 659.3 (+2) 659.2 Ac-Sox-Pro-Gly-Thr-Phe-Arg-Arg-Arg-NH2 C54H82N20O13S 22.8b 417.9 (+3) 417.9 Ac-Sox-Pro-Gly-pThr-Phe-Arg-Arg-Arg-NH2 C54H83N20O16PS 21.6b 666.3 (+2) 666.3 PKA Ac-Leu-Arg-Arg-Ala-Ser-Leu-Pro-Sox-NH2 C51H83N17O13S 25.9b 587.8 (+2) 576.8 Ac-Leu-Arg-Arg-Ala-pSer-Leu-Pro-Sox-NH2 C51H84N17O16PS 25.1b 627.9 (+2) 627.8 Abl Ac-Sox-Pro-Gly-Ile-Tyr-Ala-Ala-Pro-Phe-Ala-Lys-Lys-Lys-NH2 C79H117N19O18S 21.1c 551.6 (+3) 552.0 Ac-Sox-Pro-Gly-Ile-pTyr-Ala-Ala-Pro-Phe-Ala-Lys-Lys-Lys-NH2 C79H118N19O21PS 20.1c 578.3 (+3) 578.3
More on the same topic:
here's a list of all the assays as of 2009:
https://www.thermofisher.com/content/dam/LifeTech/global/technical-reference-library/newsletters-journals/bioprobes/pdfs/bp52/bp-52_Omnia-Kinase-assays-for-real-time-detection.pdf
notice how they list 'bulk peptide' catalog numbers? If you google these numbers, you get the likes of this:
https://assets.thermofisher.com/TFS-Assets/LSG/certificate/Certificates-of-Analysis/1640161_KNZ3071.pdf
where, lo and behold, there's a sequence at the end
Ac-EEEEYIQdP(Sox)G-NH2
Hopefully all of them still have descriptions.
Artem
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