I am dissolving the lipids in chloroform : Methanol (2:1) and drying the it to form a thin film, which is followed by overnight drying in vacuum. However next day when the liposome is re-hydrated in PBS and sonicated, it is forming a thick precipitate rather than forming a milky solution. Additionally when i am centrifuging to remove excess lipid, it its further resulting in loss of my lipid.
What is your lipid concentration and composition?? Maybe you sonicate your lipids below transitions phase temperature or something "drop out" from lipid bilayer.
I have 5mg of lipid and 0.5 mg of PEG malemide. and sonication is done keeping the eppendorf in ice
Hi
I think the composition of lipids and sonication time should be optimized for formulation, which most of the time interfere with liposomes formation.
Hi Van
I used a rotary evaporator to make the film and also used PBS at 50 degree to re hydrate my film. I am re hydrating by adding PBS glass beads and vortexing the mixture. But still finding precipitates at the end. Have previously prepared liposomes with PC by the same protocol but am not facing any such problem.