PE-DB Protein Ensemble Database - does it works?

More Pavel I. Semenyuk's questions See All

What are the secrets of preparing nickel nanoparticles?

Hi, Research gate! I need HELP! I'm trying to prepare nickel nanoparticles for my devices. I use the commonly used method from the article (DOI: 10.1002/adma.201405391). Briefly, 20 mmol...

31 March 2024 7,168 1 View

Who has researched the talent management of contests talents in mathematics, programming, etc.?

Has there been any research on managing talents who have excelled in competitions in mathematics, programming, or physics/chemistry/biology? How can we ensure that they make their most valuable...

28 February 2024 6,003 0 View

Matrigel dilution for PDX?

To what amount can you dilute matrigel? It to inject PDX subcutaneously into mouse. Undiluted it's almost impossible to work with, but overdilution leads to matrigel being incapable to solidify....

23 January 2024 6,510 2 View

Is it possible to complete a Brownian dynamics simulation of proteins via the NAMD program?

Hello. The software which is used to simulate Brownian dynamics was already discussed here...

15 January 2024 7,904 0 View

Can it be non-enzymatic digestion of BAEE in enzyme kinetics determination experiment?

Have anybody measured a kinetics of enzymatic digestion of BAEE(n-alpha-Benzoyl-Arginine Ethyl Ether) with USP method? I have observed acceptable delta-OD when use significant amounts of enzyme...

24 December 2023 9,368 1 View

What realistically maximal temperature can agarose solution reach when the tube is heated in boiling water bath?

Hello, did somebody check what could be the temperature of agarose in a tube when melted in boiling water bath?

15 December 2023 7,037 0 View

Can you provide me with an efficient plasmid extraction protocol from yeast?

I've been trying to isolate a low copy (CEN/ARS) plasmid from yeast that has selective markers for both yeast and bacteria (Trp and Amp). However, when I try to recover the plasmid I can't get any...

20 October 2023 6,749 2 View

How to compare parametric with non-parametric data?

I want to examine whether there is a significant difference between two data sets (the same number of participants, continuous variable). One has parametric distribution and another has...

06 September 2023 7,342 0 View

Pmel adoptive transfer: homozygous vs heterozygous?

Do I understand correctly that heterozygous Pmel mice have twice as less cells that carry anti-gp100 TCR? This should be right because of the allelic exclusion. If so, can one combine both homo...

05 September 2023 260 1 View

It is allowed to transform conference abstract into journal article?

I plan to present my findings on the conference. After that, I want to write a full-text article and submit it to the journal. About 90% of results will be new, while remaining 10% will be from my...

03 September 2023 4,109 3 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View