How reliable are degrees of freedom for lme mixed models in nmle package?

I am running mixed models analyses because I have participants nested in groups. Across the different analyses, I have very different degrees of freedom in the lme output with nlme package (and...

09 February 2021 6,767 3 View

Power based on past research in multilevel model?

Hi I want to replicate a published article that uses a multilevel model (participants nested into groups). I want to see the sample size I need to achieve an 80% power. I saw the simr r package...

06 January 2021 2,984 6 View

Is there an increase in protein synthesis by stress (p70-s6k)?

Hi, we are testing an antitumor drug and we have seen that the first hour increases the activation of p70-s6k but then blocks its activation. we have also found that both AKT and ERK activation...

25 October 2018 5,290 1 View

Mixed model: Level 2 IV and outcome?

Hi, I asked a related question before but I am asking a slightly different advice.. If I have a mixed model, can I do an ANOVA when both my independent variable and my dependent variable are at...

27 May 2018 6,189 9 View

Problems with flow cytometry. Cell cycle arrest by confluence?

Hello, I am testing a drug in order to see if it can stop the cell cycle. We sow 100,000 cells in a 100mm plate and treat it for 24 and 48 hours. The cells (tumoral and not tumoral) are not...

21 May 2018 6,041 2 View

SPSS factor analysis error degrees of freedom?

Hi, I'm running an exploratory factor analysis on SPSS and I have a message error: the number of degrees of freedom (0) is not positive, Factor analysis may not be appropriate. What does this mean?

10 April 2018 8,098 4 View

Does someone has the Auditory Consonant Trigram Test for children?

Hello, I was wondering is someone has access to Auditory Consonant Trigram Test for children. More specifically, I would like to have access to the protocol (e.g., trigram, time, bounds). Thank...

10 April 2018 3,660 2 View

SPSS : residual vs fit plots ?

Hi, I think I have a problem with a variable in my dataset. I have 2 dependent variables and I am regressing them on a categorical predictor. I graphed the residual vs fit plots for both of...

09 April 2018 5,547 3 View

SPSS mixed model: how to deal with a Hessian matrix error?

08 April 2018 2,698 3 View

Do you know if the CESD-R was translated in French? If so, where can I find it?

Do you know if someone has translated the French version of the CESD-R ?

02 April 2018 583 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View