Azahara Linares

3 Questions 2 Answers 0 Followers

Questions related from Azahara Linares

Our IEC-18 cells doesn´t reduce cell proliferation with LPS?

Hello, I`m testing LPS (10 ug/mL to 40 ug/mL) with IEC-18 cells and doesn´t reduce cell proliferation (MTT assay). We have changed the cell type and tested several batches of LPS and we don't...

12 May 2022 6,069 2 View

Is there an increase in protein synthesis by stress (p70-s6k)?

Hi, we are testing an antitumor drug and we have seen that the first hour increases the activation of p70-s6k but then blocks its activation. we have also found that both AKT and ERK activation...

26 October 2018 5,308 1 View

Problems with flow cytometry. Cell cycle arrest by confluence?

Hello, I am testing a drug in order to see if it can stop the cell cycle. We sow 100,000 cells in a 100mm plate and treat it for 24 and 48 hours. The cells (tumoral and not tumoral) are not...

22 May 2018 6,064 2 View