ORI Finder Tool for humans

More Sharad Ghosh's questions See All

Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View

GSH estimation assay: What is the right choice of standard?

Hi there, My question is: What standard curves should be used while estimating Tot GSH and GSSG by kinetic method using GR enzyme mediated recyling with DTNB chromophore? Actually I am following...

01 August 2024 8,217 1 View

Cell optimization in 1D Nanotubes?

Dear All, I use Quantum Espresso. I want to optimize the structure of the carbon nanotube using the vc-relax. I got confused about how to fix cell_dofree here. For 3D, cell_dofree = 'all' or...

22 July 2024 5,708 0 View

Intracerebroventricular (ICV) AAV inj.?

I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...

12 July 2024 781 0 View

What is the maximum acceptable degradation of gain in the orthogonal plane relative to the beam scanning plane for beam steering antennas?

In research papers on beam steering antennas, while the radiation pattern for the beam scanning plane (such as the horizontal or vertical plane) is typically presented, details regarding the...

03 July 2024 8,321 0 View

What kind of surface morphology must be there for good photocatalytic activity of thin film?

If I want to increase the photocatalytic activity of a thin film sample what should be it's ideal surface morphology and parameters? All scientific answers related this are highly appreciated.

19 June 2024 6,744 0 View

Why I am getting follwing error when I tried to open .dta file in SPSS25?

>Error # 7202. Command name: GET STATA >Input dictionary read error. >Execution of this command stops. Cross Product Matrices are not supported

19 June 2024 5,423 0 View

Which deposition technique is better (Spin Coating/Dip Coating) for making Ni doped ZnO thin film for better photocatalytic activity?

If I want to improve the photocatalytic activity of Ni doped ZnO thin film then which would be the better deposition process for thin film Spin Coating or Dip Coating?

18 June 2024 7,678 2 View

What are the possible parameters needed to be tuned for better photocatalytic activity of thin film?

If I want to enhance the photocatalytic activity of Ni doped ZnO thin film. For this what are the parameters I need to tune and how? Related this all scientific answers/explanations are highly...

16 June 2024 4,533 1 View

Curing of Novolak resin with hexamine develops yellow color. I believe it is due to azo linkage -C-N=N- C- What is the reaction mechanism?

Also, I will appreciate whole curing mechanism of Novolak with Hexamine

14 June 2024 9,908 0 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

Which software tools are best for enhancing diagnostic accuracy in chest X-ray imaging using image reconstruction and neural networks?

I am reaching out to seek your valuable advice and recommendations regarding the best software tools to use for this research. Specifically, I am looking for software with a user-friendly...

22 July 2024 3,794 1 View

How to use NCBI datasets ?

I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...

20 July 2024 1,339 2 View

Enquire about the calculation of percentage of reads mapped to the reference sequence?

Hello researchers, I have sequenced the A549 cell line using RNA sequencing approach and then I have it against the human genome reference sequence. Would I expect a 100% mapping percent? I got...

20 July 2024 9,705 0 View

I want to conduct a research using quasi experimental design to test two treatment and one control what type of statistical tools do l use ?

What type of experimental design do l use and what statistical tools do l use for the hypothesis. What is the position of control group in the analysis?. Thanks

18 July 2024 3,581 3 View

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

Where can I get PCR Primers for COI barcoding of birds?

We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...

16 July 2024 118 2 View

Frederic Lepretre

Hi sharad

maybe you could start with the UCSC database (https://genome.ucsc.edu). try the genome browser with hg19 as assembly, just check for your ROI, and visualize what's in the region. for memory, there are lot of track option that should interest you.

all the best

fred

Susanta Roy

I found a web tool: http://lin-group.cn/server/iOri-Human.html

Related publication: Article iOri-Human: Identify human origin of replication by incorpor...