I am a little confused on what to focus on. miRNAs are the most important, but there are longer RNAs in exosomes too... is there a way to sequence both at the same time? Or would it be better to do that separately? Why?
You can please follow the links below to obtain certain interesting informations:-
1. http://www.sciencedirect.com/science/article/pii/S1046202312001867
2. https://cofactorgenomics.com/exosome-rna-seq-primer/
3. http://www.hindawi.com/journals/bmri/2013/253957/
4. http://www.biomedcentral.com/1471-2164/14/319
Where can I find a BED file (or any other file) with all chromosomal locations of piRNAs and yRNAs in the human genome? Thanks!
02 March 2019 5,025 1 View
I understand the basic concept of quantile normalization, but am wondering how exactly that works for sequencing data. Say there are two sequencing tracks, where one has more background noise. How...
04 May 2018 2,815 0 View
Is there a reference file (bed) for enhancer regions in the mouse genome (mm9) ?
06 July 2017 2,596 1 View
I'd be very grateful for some ideas... Blood plasma was generated by differential centrifugation (200g, 2000g, and 16.000g for 15 min). Frozen at -80deg for a few days. After thawing, plasma was...
03 April 2016 3,512 4 View
I made measurements on three different days, comparing two different mouse strains. The data is noisy, but strain A was much better in a task than strain B, on all three days. However, the mean...
06 July 2014 6,196 25 View
I want to apply fluids to ex vivo samples and measure ATP in them after some time. I was wondering if I can heat the fluid samples after taking them off the tissue to say 70 deg C, to inactivate...
01 February 2014 9,348 7 View
I want to measure ATP release ex vivo, but fear that ATP is broken down too quickly. Any experiences? Thanks!
01 February 2014 1,832 1 View
I want to shake cells to mechanically stimulate ATP release, but am afraid that some cells will burst. Is there a good test to check the supernatant afterwards for that?
11 December 2013 7,480 6 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...
03 March 2021 6,193 1 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View