Does anyone know a good inhibitor to prevent extracellular ATP hydrolysis?

02 February 2014 1 2K Report

I want to measure ATP release ex vivo, but fear that ATP is broken down too quickly. Any experiences? Thanks!

You can inhibited enzyme degradation of ATP by adding EDTA. Whether your cells will stand this treatment I don't know. If not you have to separate the cells by centrifugation or filtration. Good luck!

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