I want to measure ATP release ex vivo, but fear that ATP is broken down too quickly. Any experiences? Thanks!
You can inhibited enzyme degradation of ATP by adding EDTA. Whether your cells will stand this treatment I don't know. If not you have to separate the cells by centrifugation or filtration. Good luck!
Where can I find a BED file (or any other file) with all chromosomal locations of piRNAs and yRNAs in the human genome? Thanks!
02 March 2019 5,114 1 View
I understand the basic concept of quantile normalization, but am wondering how exactly that works for sequencing data. Say there are two sequencing tracks, where one has more background noise. How...
04 May 2018 2,926 0 View
Is there a reference file (bed) for enhancer regions in the mouse genome (mm9) ?
06 July 2017 2,706 1 View
I'd be very grateful for some ideas... Blood plasma was generated by differential centrifugation (200g, 2000g, and 16.000g for 15 min). Frozen at -80deg for a few days. After thawing, plasma was...
03 April 2016 3,599 4 View
I am a little confused on what to focus on. miRNAs are the most important, but there are longer RNAs in exosomes too... is there a way to sequence both at the same time? Or would it be better to...
09 October 2015 286 1 View
I made measurements on three different days, comparing two different mouse strains. The data is noisy, but strain A was much better in a task than strain B, on all three days. However, the mean...
06 July 2014 6,306 25 View
I want to apply fluids to ex vivo samples and measure ATP in them after some time. I was wondering if I can heat the fluid samples after taking them off the tissue to say 70 deg C, to inactivate...
01 February 2014 9,442 7 View
I want to shake cells to mechanically stimulate ATP release, but am afraid that some cells will burst. Is there a good test to check the supernatant afterwards for that?
11 December 2013 7,575 6 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?
02 August 2024 9,518 2 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View