I understand the basic concept of quantile normalization, but am wondering how exactly that works for sequencing data. Say there are two sequencing tracks, where one has more background noise. How would quantile normalization work on those?
Where can I find a BED file (or any other file) with all chromosomal locations of piRNAs and yRNAs in the human genome? Thanks!
02 March 2019 5,114 1 View
Is there a reference file (bed) for enhancer regions in the mouse genome (mm9) ?
06 July 2017 2,706 1 View
I'd be very grateful for some ideas... Blood plasma was generated by differential centrifugation (200g, 2000g, and 16.000g for 15 min). Frozen at -80deg for a few days. After thawing, plasma was...
03 April 2016 3,599 4 View
I am a little confused on what to focus on. miRNAs are the most important, but there are longer RNAs in exosomes too... is there a way to sequence both at the same time? Or would it be better to...
09 October 2015 286 1 View
I made measurements on three different days, comparing two different mouse strains. The data is noisy, but strain A was much better in a task than strain B, on all three days. However, the mean...
06 July 2014 6,306 25 View
I want to apply fluids to ex vivo samples and measure ATP in them after some time. I was wondering if I can heat the fluid samples after taking them off the tissue to say 70 deg C, to inactivate...
01 February 2014 9,442 7 View
I want to measure ATP release ex vivo, but fear that ATP is broken down too quickly. Any experiences? Thanks!
01 February 2014 1,917 1 View
I want to shake cells to mechanically stimulate ATP release, but am afraid that some cells will burst. Is there a good test to check the supernatant afterwards for that?
11 December 2013 7,575 6 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View